Iran has accused the US of crossing "every limit" in international relations by expressing support for the country's anti-government protesters.
In a letter to UN officials, Iranian ambassador Gholamali Khoshroo complained that Washington was intervening "in a grotesque way in Iran's internal affairs".
He said US president Donald Trump's "absurd tweets" have encouraged disruption.
The ambassador said Mr Trump and vice-president Mike Pence were personally stirring up trouble.
"The president and vice-president of the United States, in their numerous absurd tweets, incited Iranians to engage in disruptive acts," the ambassador wrote to the UN Security Council president and UN secretary-general Antonio Guterres.
The US did not immediately respond to the letter, which maintains that Washington "has crossed every limit in flouting rules and principles of international law governing the civilised conduct of international relations".
At least 21 people died and hundreds were arrested a week of anti-government protests and unrest over economic woes and official corruption.
Meanwhile, tens of thousands of people took part in counter-demonstrations on Wednesday backing the clerically overseen government, which has said "enemies of Iran" are fomenting the protests.
Mr Trump has unleashed a series of tweets in recent days backing the protesters, saying Iran is "failing at every level" and declaring that it is "time for change" in the Islamic Republic.
"Such respect for the people of Iran as they try to take back their corrupt government," he tweeted on Wednesday. "You will see great support from the United States at the appropriate time!"
Mr Trump's UN envoy, ambassador Nikki Haley, called on Tuesday for an emergency Security Council meeting on Iran, saying the UN needed to speak out in support of the protesters.
As yet, no meeting has been scheduled.
Mr Guterres is following the developments in Iran with concern, deputy spokesman Farhan Haq said earlier on Wednesday.
He said the secretary-general urged respect for free-expression rights and stressed that any demonstrations should be peaceful.
At most some 42,000 people took part in the week of protests that rocked the Islamic Republic, Iran's interior minister has said.
Abdolreza Rahmani Fazli said in a statement on Thursday that the figure was "based on precise statistics we have".
Mr Fazli said the continuation of the protests during the past week was because of the "leniency, restrain, tolerance and interaction" of the government.
He did not elaborate.
This is the first time authorities have given a figure for the total number of participants in the protests.
On Wednesday, General Mohammad Ali Jafari, the chief of Iran's paramilitary Revolutionary Guard, said the biggest gathering included some 1,500 protesters.
The protest began on Thursday last week in Mashhad and quickly extended to other cities.
