Flying Scotsman was involved in a “slow speed” crash with another heritage train hours before visitors were due to board it.
The crash happened at Aviemore Railway Station, in the Cairngorms, Scottish Highlands, at 7.10pm on Friday.
The station is home to Strathspey Railway, a heritage line which takes visitors on steam trains in northern Scotland.
Two people were taken to hospital as a precaution but their injuries were not believed to be serious, police said.
A spokesman for the National Railway Museum said: “We can confirm that steam locomotive Flying Scotsman has been involved in a shunting incident at Aviemore Station.
“We will provide further information once more details are known.”
Scottish Fire and Rescue Service (SFRS) sent eight appliances to the scene, and Police Scotland said other casualties were treated at the station.
Ariane Burgess, Scottish Greens MSP for Highlands and Islands (Region), said: “We are still waiting on further information about what exactly has happened to cause this, but I understand people were injured and my thoughts are with them.
“We are indebted to the emergency services and rail and train crews who have been working to help and treat the casualties and others at the scene, and hospital teams dealing with them. They have our heartfelt thanks.
“I am seeking further information on what has occurred and will be following details closely.”
Scottish Greens transport spokesperson Mark Ruskell MSP said: “It is too early to know what has caused this incident, but clearly a full investigation will have to take place.
“From reports it appears this has been on the heritage track, the Strathspey Railway line, involving the Flying Scotsman and that nearby services on the main line have been temporarily impacted to allow emergency efforts to continue.
“We are thinking of all those involved at this challenging time and thank all those who have taken part in the emergency response.”
A spokesman for SFRS said: “We were alerted at 7.10pm on Friday to reports of a collision involving two trains at Aviemore Railway Station.
“We sent eight appliances to the scene, where firefighters worked to support partners.
“Three appliances remain at the scene.
“Two casualties have been transported to Raigmore Hospital.
“Three casualties were treated at the scene but did not require to be taken to hospital.”
A Police Scotland spokesperson said: “Around 7pm on Friday September 29, we received a report of two trains colliding at low speed at Aviemore Railway Station.
“As a precaution, two people – a man and a woman – were taken to Raigmore Hospital in Inverness by the Scottish Ambulance Service for treatment. Their injuries are not believed to be serious.
“A number of other passengers were assessed by the ambulance service at the scene and did not require hospital treatment.
“Inquiries are ongoing to establish the full circumstances.”
Flying Scotsman was scheduled to be running trips this weekend, with the line expected to be especially busy.
A spokesman for Network Rail said: “It’s on a heritage railway line, it’s not our infrastructure.
“The main line is shut while emergency services respond.
“We are saying to passengers to check before travelling.”
The Strathspey Railway was contacted for comment.
