The Orange Order has been banned from marching along the mainly Catholic Garvaghy Road this weekend.
Members wanted to parade through the nationalist district of Portadown to mark 25th anniversary since they were last allowed to make their way from Drumcree Church.
The dispute began in 1995 when nationalists blocked the return route of an Orange Order parade.
After a three-day stand-off, the parade finally went ahead after nationalists agreed to end their protest.
A year later the parade was banned but after violent clashes between the RUC, Orangemen and their supporters, the decision was reversed, resulting in nationalist protesters being forcibly removed from the Garvaghy Road.
At the height of the stand-off, Catholic taxi driver Michael McGoldrick (31) was shot dead by the UVF outside Lurgan.
In 1997 the Orange Order was again allowed to march along the Garvaghy Road after the area was flooded by police in the early hours of Drumcree Sunday.
Read more:What is the Orange Order? An explainer
Priests were forced to celebrate Mass in front of British army lines after local people were blocked from attending their local church.
The dispute around the parade, which was eventually banned in 1998, was also linked to a deadly sectarian arson attack carried out by the UVF in the early hours of July 12 1998.
Catholic schoolboys Richard (10), Mark (9) and Jason Quinn (8) died after their home was petrol bombed in Ballymoney, Co Antrim.
While some Orangemen continued their ill-fated protest, others left Drumcree and have not returned.
While most nationalists consider the dispute to be at an end, and many young people have no memory of an Orange parade in their area, some Orangemen continue to insist they be allowed through the Garvaghy district.
The Orange Order had wanted to bring 1,500 members and a similar number of supporters onto the road this year to mark the 25th anniversary of the original ban.
In a determination issued on Thursday, the Parades Commission put the brakes on any prospect of the parade taking place along the route.
In representations to the commission, the order said the "route was categorically not negotiable".
In its representations, the Garvaghy Road Residents Coalition described the impact of the past on the local community and "the trauma that remains".
It added that any initiative to facilitate a march on the road would bring to the fore "issues, anxieties and fears which the residents consider should remain in the past and which could have wider ramifications".
Residents also signalled their willingness to accept "an alternative, less contentious route along Corcrain/Dungannon Road".
Speaking to The Irish News, spokesman Breandán MacCionnaith said a number of concerns were raised including the proposed attendance of an additional two flute bands.
Concerns over access to Mass at a nearby Catholic church around the time of the planned parade have also been raised.
He added that young people from the area have "never had any experience of a parade along the Garvaghy Road" or nearby Obin Street, which was a parading flashpoint in the 1980s.
"By and large the parade out to Drumcree is largely ignored," he said.
"But obviously given that the Orange Order themselves are beefing this up into 25 years, and with the backing of the grand lodge, we will just have to wait and see what happens on Sunday week."
"There's nobody in our community making any noises or talking about marking 25 years of no marches."
Meanwhile, prominent loyalist Jamie Bryson has criticised the Parades Commission decision to ban the parade and described the dispute as "unfinished business" on Twitter.
"Drumcree is unfinished business, and the issue should be forced by the loyal orders."
